Journal of the Korean Society of Cosmetology 2011;17(4):727-735.
Published online August 31, 2011.
두피질환과 모발표면에 오염된 미생물의 상관관계
김관옥, 김성남, 박두현
Research Paper : Relationship Between Scalp Disorder and Bacterial Community Contaminated Hair
Kwan Ok Kim, Sung Nam Kim, Doo Hyun Park
Abstract
This research was performed to analyze and compare the diversity of bacterial community contaminated hairs of women whose scalp was diseased or healthy. It is possible that the physical conditions of scalp may be a factor to influence bacterial community diversity contaminated hair surface. Hairs were sampled from 10 women whose scalp was healthy and other 10 women whose scalp was slightly diseased. 16S-rDNA was amplified with chromosomal DNA template extracted from the hairs sampled from 20 women. 16S-rDNA variable region was amplified selectively to analyze by temperature gradient gel electrophoresis (TGGE). Maximal 8 DNA bands and minimal 4 DNA bands were separated by TGGE performed with 16S-rDNA obtained from 10 of the healthy scalp hairs; meanwhile, maximal 11 DNA bands and minimal 4 DNA bands were separated by TGGE performed with 16S-rDNA obtained from 10 of the diseased scalp hairs. Sequences of DNAs extracted from the TGGE bands were performed and identified based on the sequence homology. The diversity of bacterial species contaminated the diseased scalp hairs was higher than that contaminated the healthy scalp hairs. Conclusively, the physical condition of the diseased scalp is supposed to be a cause to increase relatively opportunity for bacterial contamination of hairs.
Key Words: Scalp, Bacterial contamination, Hair, TGGE, 16S-rDNA


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