| 고지방식 유도 비만마우스에서 간 및 지방조직의 변화 관찰을 중심으로 한 꾸지뽕 에탄올추출물의 항비만 효과 |
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이채명, 김배환 |
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| Research Paper : The Anti-obesity Effect of Cudrania Tricuspidata Ethanol Extract by Focusing on the Changes of Hepatic and Adipose Tissue in HFD-induced Obese Mice |
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Chae Myeong Lee, Bae Hwan Kim |
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| Abstract |
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In order to evaluate the anti-obesity effect of Cudrania tricuspidata Bureau ethanol extract in HFD-induced obese mice, we conducted histological and molecular biological experiments. The thirty-five, six-week-old C57BL/6 male mice were classified into five groups (7 in each group) such as normal group (N), control group (C), positive control group (PC, 0.5% catechin), experimental group 1 (E1, 2% Cudrania tricuspidata Bureau stem ethanol extract, CTSEE) and experimental group 2 (E2, 2% Cudrania tricuspidata Bureau leaves ethanol extract, CTLEE). After inducing the obesity by providing high-fat diet except normal group for four weeks, we provided catechin, CTSEE and CTLEE for additional five weeks. Daily body weight gain significantly decreased (p<0.05) in E1 group, compared to C group. In histological observation (H&E staining) of hepatic tissue, the lipid deposit of E1 and E2 group was decreased compare to C group. The size of epididymal adipose tissue cells decreased (p<0.05) significantly in E1 group, compared to C group. In antioxidant activities of hepatic tissue, there were significant increases (p<0.05) of SOD and CAT, and significant decreases (p<0.05) of XO and TBARS in PC, E1 and E2 group, compared to C group, which indicated catechin, CTSEE and CTLEE showed superior antioxidant ability. The mRNA expression related to the lipid metabolism was evaluated in hepatic tissue and epididymal adipose tissue. There were significant decreases (p<0.05) of acetyl-CoA carboxylase (ACC) and peroxisome proliferator activated receptor- |
| Key Words:
Anti-obesity, Cudrania tricuspidata ethanol extract, ACC, PPAR-γ, adiponectin |
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