Journal of the Korean Society of Cosmetology 2018;24(6):1306-1315.
Published online December 31, 2018.
para-Phenylenediamine처리에 따른 HaCaT 세포주의 세포변성효과 및 생존율
홍은설, 송연숙, 이근광
Cytopathic Effect and Cell viability on the HaCaT Cell Line of para-Phenylenediamine of Hair Dye Ingredients
Eun-seoul Hong, Yeon-sook Song, Keun-kwang Lee
Abstract
The purpose was to investigate the cytopathic effect and cell viability on cultivated HaCaT cell lines after processing for 24 hours and 48 hours through treatment with differing concentrations of PPD. The results are as fellows: Normal HaCaT cell lines grew normally in a cell cultivation flask, and were almond shaped. HaCaT cell lines that were exposed to the toxicity of PPD were generally inactive and showed signs of the cytopathic effect because of weak adhesion. Less than 50% cell viability was observed after 50 minutes in a group with 0.5% PPD, About 48% cell viability was observed after 10 minutes in a group with 1~3% PPD, and more than 45~40% cell viability was observed after 10 minutes in a group with 4~5% of PPD. The results due to the degree of cytotoxicity show that; The survival rate of cultivated cells after 48 hours increased after treatment for 10 minutes for a group with 0.5~1% PPD compared to cultivated cells treated for 24 hours. This suggests cells recovered little by little after 48 hours from a group with less than 1% of the treatment concentration for 10 minutes. But, they didn
Key Words: Cell viability, cytopathic effect, HaCaT cell line, para-Phenylenediamine, PPD
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