미강(Rice Bran) 에탄올 추출물의 항산화 활성과 티로시나제 저해 활성 |
전선복, 전정애, 정병구 |
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Research Paper : Anti-oxidative Activities and Tyrosinase Inhibition Ability of Rice Bran Ethanol Extract |
Seon Bok Jeon, Jeong Ae Jeon, Byeong Goo Jeong |
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Abstract |
In this study Rice Bran was used for investigating the anti-oxidative activities and skin whitening effects. Rice Bran extract prepared with ethanol. Inhibitory effect of Rice Bran Ethanol Extracts (RBEE) onto free radical generation was determined by measuring DPPH scavenging activities in vitro. Intracelluar oxidation was determined by DCF-DA assay. As a result, anti-oxidant activity is in a dose dependant manner. RBEE showed 94% of free radical scavenging activity on DPPH at a concentration of 500ug/ml compare to control. Human dermal fibroblast (HDF) cells incubated with RBEE showed reduces ROS effectively. The inhibitory action of RBEE on mushroom tyrosinase was revealed. Tyrosinase inhibitory activities of RBEE showed a dose response relationship. RBEE showed 21% inhibitory effect on tyrosinase activity in the mushroom tyrosinase of 20ug/ml and RBEE showed effectiveness in inhibiting L-DOPA auto-oxidation. On the other hand, inhibitory activity of tyrosinase were lower than that of Kojic acid. These results suggest that Rice Bran may prevent skin aging and coloring via anti-oxidative effect and inhibitory activity of tyrosinase. |
Key Words:
Rice bran, Anti-oxidative, DPPH Radical, Tyrosinase |
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