| 마우스대식세포에서 천연복합혼합물의 항산화 및 항염 효과 |
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이은경, 정숙희 |
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| Anti-inflammatory and Anti-oxidant effect of Natural Herbs Mixture in RAW264.7 Cell Line |
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Eun-kyung Lee, Sook-heui Jung |
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| Abstract |
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Recently, there have been various studies on the efficacy of complex extracts in which various components are incorporated into cosmetics rather than the effects of single extracts. The anti-oxidative and anti-inflammatory effects of mixture were investigated in vitro for use as cosmetic materials. Green tea (GT) by supercritical CO2 extraction, Oenanthe javanica (OJ), Perilla frutescens var (PF), Zanthoxylum piperitum (ZP) by steam distillation, Burdock (Arctium lappa L.) (BA), Orostachys japonicus A. Berger (OA), Puerariae Radix (PR) by ethanol were mixed same ratio (HM). MTSassay was used to confirm the toxicity of RAW 264.7 cells. When the survival rate of the control was taken as 100%, the cell survival rate of individual extracts was 87% or more at 1, 10 and 100 ug/ml, the cell survival rate of 94% or more was confirmed at 50, 250, and 500 ppm of HM. The DPPH radical scavenging activity of the extracts showed the highest level of 90.47% in the ZP extracts. HM showed anti-oxidative effect of 92.47% EDA similar to Ascorbic Acid. Inhibition of NO production and inhibition of TNF- |
| Key Words:
Anti-inflammatory, Anti-oxidant, Nature complex mixture |
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